Biology: Insulin.

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BRIEF INTRODUCTION

Insulin is a protein hormone secreted from the Beta cells in the pancreas. Insulin is produced when there is a high level of sugar in the blood, insulin acts on cells in the body to help reduce blood sugar levels. Diabetics, who suffer from lack of insulin production, must rely on technology to produce insulin. Using Recombinant DNA Technology is one way artificial insulin can be produced. The processes for isolating and producing large amounts of insulin require specific enzymes, other bacteria and of course sophisticated technology. In this project, we 'll be discussing terms such as Restriction Enzymes they are specific proteins that speed up chemical reactions. Providing the right conditions they 'cut' DNA strands at specific locations, usually between the Guanine (G) and Adenine (A) bases. Restriction enzymes recognise specific sequences of DNA, and cut at these points. Similarly, another enzyme, called DNA Ligase, will also be discussed. The enzyme Ligase joins pieces of double stranded DNA at their sugar-phosphate backbones. The bonds that form here are strong (covalent) bonds. When isolating and cloning the gene - ligase, restriction enzymes and a vector, such as a plasmid found in bacteria, are used. A Vector (plasmid), is a small circular piece of DNA found in bacteria. Because bacteria multiply in large amounts by binary fission, they're used to manufacture and clone large amounts of insulin in a short amount of time, as the vector will have the insulin gene inserted. Thus cloning the insulin gene also. JOINING DNA FRAGMENTS

In gene manipulation, the joining of pieces of DNA with sticky ends to make one circular piece is sometimes required. The enzyme LIGASE (pic 71) joins pieces of double stranded DNA at their sugar-phosphate backbones. The bonds that form are strong (covalent) bonds.

TRANSPORTING DNA INTO CELL

School papers on Biology: Insulin.

Special carriers or vectors can transport DNA fragments into cells, such as bacteria. One example of a vector is a small circular DNA molecule, called a plasmid, found in bacteria. Under certain conditions plasmids can cross cell membranes and enter a cell. When it moves from outside to inside, it carries any passenger DNA which has been joined to it.Please note that this sample paper on Biology: Insulin. is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Biology: Insulin., we are here to assist you. Your cheap custom college papers on Biology: Insulin. will be written from scratch, so you do not have to worry about its originality.

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Thomas Jefferson and Slavery

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The declaration of independence has been the symbol of American peoples desire to implement democracy in its true form. When Thomas Jefferson wrote all men are created equal, he introduced the ideology of equal opportunity for everybody in pursuit of their individual goal. This country was founded on the principle of equality among the people. The practice of this ideology is what set the American government apart from other governments at the time. However the definition of all men is not as clear as it seems. There have been a lot of discussions about Jeffersons intention when he wrote this famous statement. When examining the conditions that were present at the time, we can say that Jeffersons vision of equality was not inclusive of everyone.

In 1776, slavery was a common practice in United States. The economy was largely dependent on the institution of slavery. The slaves were dark skinned people who were brought from Africa. The difference in the way the look and the color of their skin made them to be outcast. They were given the lowest position in the society. The slaves performed most of the labor work that was considered inappropriate for any white person. The general public held an opinion about blacks to be from an inferior race. There were many people who thought of blacks not to be human beings.

Thomas Jefferson was born into a rich family who had the privilege to own slaves. When he became adult, he inherited his family estate and became a slave owner. In fact, the prosperity of his estate was very much dependent on the slaves he owned. He spent his whole life in the midst of slaves over the course of his lifetime. Surely he was well aware of the sub-human treatment that the slaves were subjected to. It is fair to say that Jefferson was not as much concerned about the inequality that existed between blacks and whites as he was about his economic situation.

During Jeffersons time of public service, he was known to be against slavery. He believed that the institution of slavery violated the core principle of the American Revolution. When he drafted the Declaration of Independence, he inserted a paragraph that calls for the people to abolish slavery, which was taken out by other member of the congress before it was signed. However, his view of anti slavery did not mean he regarded the blacks as equal. In fact, in one of his writings, he sated that blacks are biologically inferior to whites, and he went on to say that if slaves were to become free, they should be separated from whites in order to avoid racial mixing.

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Duplicity of Lady Macbeth

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Lady Macbeth is highly duplicitous in the play Macbeth.

Although she appears to be completely inoccent.It is in fact her,rather than Macbeth,who is most to blame for the Murder of King Duncan and the turmoil which follows.It is her greed which powers the wishful thinking that Macbeth experiences when he hears the predictions of the witches at the start of the play.This will be later examined in my essay entitled What powers Macbeth?In this essay I will strive to show the true colours of Lady Macbeth and how in fact Macbeth and Lady Macbeth are equally to blame for their downfall if not that she is to blame more.For example, Macbeths guilt at the end of the book is visible in his consious mind (when he is awake) , this shows that he shows real guilt and wants to tell someone so that he can cleansed of his sins.Whereas,with Lady Macbeth,her guilt is only in her subconcious,her conscience interfering only when she is asleep.This shows that she does not wish anyone to find out what she has done but only to hide it.It is ironic that her trying to conceal the problem means that it is conveyed to her nurse through her sleep-talking.She maintained her cool whilst talking to Macbeth only to keep him silent by saying that she felt no guilt, although evidently she did.Macbeth is a play based on a real king who was nothing like the character in the play. It is unfair that scottish history be defaced in this way by Shakespeare.But did Shakespeare in fact write his plays.In the novel Flowers for Algernon the main character Charlie challenges this.Wsa it not that Shakespeare was illiterate.He surely could not read and write. Maybe someone else , a scotsman hating english king wrote Macbeth or maybe an it was indeed an angry Shakespeare.U must remember that to understand a novel u mist first understand its author.I must do research on the most famous English playright before I can truely understnad who Lady Macbeth was based on, if anyone at all.Could it be Shakespeares mother,sister, a girl on the street, school mistress, a real queen?

Please note that this sample paper on Duplicity of Lady Macbeth is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Duplicity of Lady Macbeth, we are here to assist you. Your cheap research papers on Duplicity of Lady Macbeth will be written from scratch, so you do not have to worry about its originality.

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Music

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Something that I have always felt very passionate about is music. Music is the only thing that has been with me through everything. It has helped shape me into the person I am today. Who I would be without music in my life, I don't know, and honestly, I don't care to know. Music has been perfect for every situation in my life so far. It can make me feel happy, excited, or relaxed.

When I've had a bad day, and I just want to cheer up, all I have to do is turn on my Blind Melon or Tom Petty CD. This music never fails to put a smile on my face. It literally makes my head feel lighter, makes me breath a little deeper, and gives me the feeling of walking on air. If I close my eyes and concentrate on the lyrics, the worries of my day just float right out of my head. Tom Petty brings back so many memories of nights out with the girls when we drove around singing as loud as we could. Those were such fun times, every time I listen to Tom Petty's songs I just get happy. Blind Melon's lyrics are so true to life. Their music makes me realize a lot; it's very refreshing to me.

When I'm getting ready to meet friends to go out and have fun, I always give myself a little kick by playing some Led Zeppelin or The Doors. The sounds that come from a guitar absolutely amaze me. Nothing gets me going more than listening to the amazing music by Led. It always makes me want to dance around and sing really loud. Music by The Doors just speaks to me. I have always been a huge fan of this group. Their music blows me away. When I turn on The Doors and let Jim Morrison's voice sink into my head, I can't do anything but get excited. After hearing the first note of his voice, I am always ready to get out and have a good time.

When I come home from school ready to rip all of my hair out, I try to keep myself under control until I can reach my CD player. Any time I'm stressed out or tense, all I need to relax is a good Pink Floyd song. I grew up listening to this music, so it puts me at ease. Pink Floyd's music and lyrics are so intense, I don't even have to think about anything. I just lay back and let the sounds float in my head. His smooth voice has served as my everlasting backrub on countless occasions. If I feel uncomfortable in any way, and I need to calm down and relax, The Beatles will do the trick within seconds of pushing the play button. My dad has always loved The Beatles, and played them constantly during my childhood. I think songs by this group are the songs I've heard the most in my life. They are so familiar; their music makes me feel very comfortable. I feel so at home when I'm listening to them, like I'm exactly where I need to be. Music by The Beatles is so comforting to me, they will put my right to sleep.

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The Wild Goose Chase

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The Wild-Goose Chase My Description and Opinions on Annie Dillards The Chase

Dillard's essay tells the story of an event in her childhood during winter when she was seven. Before describing this she gives us a brief history of herself as a child. She used to play both football and baseball with the boys during the summer. These boys were slightly older than Dillard and she seems to have prided herself on being as rough and tough as any of them. When it was too cold for football and baseball and there was enough snow on the ground, she would play snowball fights with the boys instead. Since she describes herself as having a "boy's arm" (7) for throwing baseballs, I imagine she was also formidable with a snowball in hand. In the event she describes in the essay she was with a group of boys waiting to throw their snowballs at passing cars. Then when one heralded its approach by the sound of snow chains clanking on the pavement, they readied themselves to pelt it with snow as normal. But, when they let loose, the driver pulled over and ran toward them. Soon the group of children split off and ran in different directions and Dillard was left being chased by this man with another boy ahead of her. Though she expected this driver to give up after scaring the children into fleeing he carried on running them down. He chased them over and around ten blocks before he caught them and at that point had to wait awhile to catch his breath before he could even start to speak. Then, with his suit covered in snow, he proceeded simply to tell them off. For a seven-year-old this was a huge anticlimax after the excitement of a chase all that happened was a routine dressing down.

I really like this essay. It's a very good description of the thoughts and feelings going through a child's mind. Which is an amazing feat considering the author wrote the essay many years after the fact. Though being chased by this man was frightening it obviously was also an exciting and exhilarating experience for her as well. She describes what happens as if it's a victory for her as a child, she got a kick out of being chased and all the man who chased her could do was scold a couple of little kids who aren't listening to him. Though at the same time she admired this man, a "sainted, skinny, furious redheaded man" that "would never give up" (100). She admired him for knowing "what I thought only children . . . knew" that you had to "fling yourself at what you're doing" () with the singlemindedness of a child. Write a research paper on The Wild Goose Chase on The Wild Goose Chase will be written from scratch, so you do not have to worry about its originality.

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